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Rules to improve packing structures used in liquid chromatography

Friday, 23 May, 2008 - 17:00
Campus: Brussels Humanities, Sciences & Engineering campus
Jeroen Billen
phd defence

High performance liquid chromatography (HPLC) is one of the most successful an widely used analytical separation tools. Most analytical laboratories, at some point in their research activities, rely on HPLC. Although the technique has come to maturity, there is still a demand coming from different scientific domains for better performing HPLC columns and instruments that can handle faster and/or more complex separations.

Several routes for the further optimization of HPLC have opened up to explore. The scope of the thesis was to address the feasibility of these different optimization strategies. The research activities can be divided into two main sections. In the first part computational methods were used to gain more theoretical insight in the band broadening sources determining the quality of a chromatographic separation. In the second part, these acquired theoretical insights were employed to study new evolutions emerging in modern day HPLC. The goal was to provide users as well manufacturers with a number of straightforward rules on how to improve their separations by the adjusting the operating parameters and/or optimizing the column structures. Applying these rules would make it possible to determine the full potential of a chromatographic system, compare different support structures with each other and improve them.