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Understanding the effects of velocity, pressure, temperature and composition gradients in HPLC

woensdag, 26 mei, 2010 - 17:00
Campus: Brussels Humanities, Sciences & Engineering campus
Faculteit: Arts and Philosophy
Ken Broeckhoven

The constant demand from industry and research laboratories for faster and more
efficient separations to analyze complex samples has led to the development of highly
efficient instruments capable of operating at ultra-high pressures (> 1000 bar) using
micron sized particles. However, with the ever increasing separation quality, secondary
effects, that were up to now invisible in the overall observed efficiency, become more
and more pronounced. These effects limit the ultimate separation performance which
can be achieved. Their origin is found in local differences (also know as gradients) of,
for example, the analyte concentration, the velocity of the mobile phase, or the
temperature of the separation medium. In this thesis, a detailed analysis was made of
the origin, amplitude and time dependency of these effects in both current and future
chromatographic systems. In addition, the influence of deliberate changes in the
operating conditions, e.g., by applying mobile phase gradients, is studied as well.